This study aimed to assess the knowledge and utilization of ultrasound scans among pregnant women attending antenatal clinics at Orile-Agege General Hospital, Lagos State. The objectives were to evaluate their awareness and usage of ultrasound scans and identify the challenges affecting their utilization. A convenience sampling technique was used to select 125 pregnant women, out of which 122 were validated for the study. Data were collected using a selfdeveloped questionnaire and analyzed using frequency/percentage tables and charts with SPSS version 25 and Microsoft Excel 2010.The findings indicated a generally fair level of knowledge and utilization of ultrasound scans among participants. The key challenges to ultrasound scan utilization included lack of spousal or family support (57.4%), cultural beliefs and societal norms (37.7%), cost of scans (31.1%), limited access to ultrasound services (26.2%), lack of awareness (16.4%), and long waiting times at diagnostic centers (13.1%).Furthermore, the study established a significant relationship between participants' educational levels and their knowledge of ultrasound scans (p < 0.05). However, no significant relationship was found between their knowledge and actual utilization of ultrasound scans (p > 0.05).