The objectives of this study were: (1) to find out the extent to which, if at all, a relationship exists among transformational leadership components, job satisfaction, and academic service quality among public university staff in Cambodia; and to determine the extent to which, if at all, differences in university staff demographic characteristics (gender, age, current position, and working experiences) are associated with the degrees of transformational leadership, job satisfaction, and academic service quality among public university staff in Cambodia. A sample of 336 staff from three selected public universities located in Phnom Penh completed the Multifactor Leadership Questionnaire (MLQ) to measure transformational leadership dimensions, the Minnesota Satisfaction Questionnaire (MSQ) to measure the level of satisfaction, the Service Quality (SERVPERF) to measure academic service quality, and a demographic survey. Collected data were analyzed using the descriptive and inferential statistics. The first research question hypotheses were tested using Pearson’s product-moment correlation coefficient at a significance level of p <.01, and the second research question hypotheses were tested using multivariate analysis of variance at a significance level of p <.05. This study found that there were favorable correlations between transformational leadership dimensions, job satisfaction and academic service quality, as well as job satisfaction and academic service quality among staff at three selected public universities in Phnom Penh. Regarding the demographic data and university staff perceptions, the selfperceived levels of transformational leadership and academic service quality were not influenced by gender, current positions, or work experiences. However, the perceived levels of job satisfaction and individual consideration among university staff were observed to fluctuate according on age. The findings of this study offer empirical research on this subject in higher education, which advances the field of leadership studies